A non-animal new approach methodology to test predict human blood toxicity like safety concerns

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Catalogue Name :
HeMOToX
Cell Type/ Tissue:
TRANS-HSC

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A non-animal new approach methodology to test predict human blood toxicity like safety concerns

Cell type used as testing platform
TRANS-HSC: Characterized Configured Human hematopoietic stem cell type of adult primary and progenitors
About Hematotoxicity
Any drug, compound agent or perturbation, (drug, environmental agent or xenobiotic, food addition, cosmetic addition, fuel addition, radiation or other exposure perturbation), has the potential to affect the normal steady state functioning and regulation of the blood-forming or hematopoietic system.
The hematopoietic system, like most biological systems in the body, is a stem cell system. The hematopoietic system is unique, however, in that it has the potential to produce multiple cell types, each with different functions, from a single stem cell. For all biological systems, the stem cell, or rather the stem cell compartment, which contains many different stages of stem cell primitiveness or maturity, is the foundation upon which all biological systems are built.

Assay endpoints:
Colony Forming Assay
CFC assay does quantitatively measure cell proliferation, but also determines the ability of the cells to differentiate. The CFC assay is whole dependent upon the cells in the colony being able to differentiate and mature so that the colonies can be identified as belonging to one or another hematopoietic lineage. In this way, the possibility of an agent causing neutropenia can be differentiated from that causing anemia or thrombocytopenia.

Reference:

Avila AM, Bebenek I, Bonzo JA, Bourcier T, Davis Bruno KL, Carlson DB, Dubinion J, Elayan I, Harrouk W, Lee SL, Mendrick DL, Merrill JC, Peretz J, Place E, Saulnier M, Wange RL, Yao J, Zhao D, Brown PC. An FDA/CDER perspective on nonclinical testing strategies: Classical toxicology approaches and new approach methodologies (NAMs). Regul Toxicol Pharmacol. 2020 Jul;114:104662. doi: 10.1016/j.yrtph.2020.104662. Epub 2020 Apr 20. PMID: 32325112.

Mahalingaiah PK, Palenski T, Van Vleet TR. An In Vitro Model of Hematotoxicity: Differentiation of Bone Marrow-Derived Stem/Progenitor Cells into Hematopoietic Lineages and Evaluation of Lineage-Specific Hematotoxicity. Curr Protoc Toxicol. 2018 May;76(1):e45. doi: 10.1002/cptx.45. Epub 2018 Apr 30. PMID: 30040226.

Available Resources

Catalogue Name :
HeMOToX
Cell Type/ Tissue:
TRANS-HSC

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